PubMed İndeksli Yayınlar Koleksiyonu

Permanent URI for this collectionhttps://hdl.handle.net/20.500.12294/2425

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Agglomerated Serum Albumin Adsorbed Protocatechuic Acid Coated Superparamagnetic Iron Oxide Nanoparticles as a Theranostic Agent
(IOP Publishing Ltd, 2023) Bozoglu, Serdar; Arvas, Melih Besir; Varli, Hanife Sevgi; Ucar, Burcu; Acar, Tayfun; Karatepe, Nilgun
Iron oxide nanoparticles have been one of the most widely used nanomaterials in biomedical applications. However, the incomplete understanding of the toxicity mechanisms limits their use in diagnosis and treatment processes. Many parameters are associated with their toxicity such as size, surface modification, solubility, concentration and immunogenicity. Further research needs to be done to address toxicity-related concerns and to increase its effectiveness in various applications. Herein, colloidally stable nanoparticles were prepared by coating magnetic iron oxide nanoparticles (MIONPs) with protocatechuic acid (PCA) which served as a stabilizer and a linkage for a further functional layer. A new perfusion agent with magnetic imaging capability was produced by the adsorption of biocompatible passivating agent macro-aggregated albumin (MAA) on the PCA-coated MIONPs. PCA-coated MIONPs were investigated using infrared spectroscopy, thermogravimetric analysis and dynamic light scattering while adsorption of MAA was analysed by transmission electron microscopy, Fourier-transform infrared spectroscopy and x-ray diffraction methods. Magnetic measurements of samples indicated that all samples showed superparamagnetic behaviour. Cytotoxicity results revealed that the adsorption of MAA onto PCA-coated MIONPs provided an advantage by diminishing their toxicity against the L929 mouse fibroblast cell line compared to bare Fe3O4.
Host-Guest Interactions of Caffeic Acid Phenethyl Ester With β-Cyclodextrins: Preparation, Characterization, and in Vitro Antioxidant and Antibacterial Activity
(Amer Chemical Soc, 2024) Acar, Tayfun; Arayici, Pelin Pelit; Ucar, Burcu; Coksu, Irem; Tasdurmazli, Semra; Ozbek, Tulin; Acar, Serap
The aim of this study is to improve the solubility, chemical stability, and in vitro biological activity of caffeic acid phenethyl ester (CAPE) by forming inclusion complexes with beta-cyclodextrin (beta-CD) and hydroxypropyl-beta-cyclodextrin (H beta-CD) using the solvent evaporation method. The CAPE contents of the produced complexes were determined, and the complexes with the highest CAPE contents were selected for further characterization. Detailed characterization of inclusion complexes was performed by using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and electrospray ionization-mass spectrometry (ESI-MS). pH and thermal stability studies showed that both selected inclusion complexes exhibited better stability compared to free CAPE. Moreover, their antimicrobial activities were evaluated against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) for the first time. According to the broth dilution assay, complexes with the highest CAPE content (10C/beta-CD and 10C/H beta-CD) exhibited considerable growth inhibition effects against both bacteria, 31.25 mu g/mL and 62.5 mu g/mL, respectively; contrarily, this value for free CAPE was 500 mu g/mL. Furthermore, it was determined that the in vitro antioxidant activity of the complexes increased by about two times compared to free CAPE.
Synthesis of Novel Coumarin-Triazole Hybrids and First Evaluation of the 4-Phenyl Substituted Hybrid Loaded PLGA Nanoparticles Delivery System to the Anticancer Activity
(Iop Publishing Ltd, 2024) Arvas, Busra; Ucar, Burcu; Acar, Tayfun; Varli, Hanife Sevgi; Arvas, Melih Besir; Aydogan, Feray; Yolacan, Cigdem
Despite the discovery of many chemotherapeutic drugs that prevent uncontrolled cell division processes in the last century, many studies are still being carried out to develop drugs with higher anticancer efficacy and lower level of side effects. Herein, we designed, synthesized, and characterized six novel coumarin-triazole hybrids, and evaluated for anticancer activity of the one with the highest potential against the breast cancer cell line, MCF-7 and human cervical cancer cell line, human cervical adenocarcinoma (HeLa). Compound 21 which was the coumarin derivative including phenyl substituent with the lowest IC50 value displayed the highest cytotoxicity against the studied cancer cell line. Furthermore, the potential use of poly (lactic-co-glycolic acid) nanoparticles (PLGA NPs) prepared by the emulsifying solvent evaporation method as a platform for a drug delivery system was studied on a selected coumarin derivative 21. This coumarin derivative-loaded PLGA NPs were produced with an average size of 225.90 +/- 2.96 nm, -16.90 +/- 0.85 mV zeta potential, and 4.12 +/- 0.90% drug loading capacity. The obtained 21-loaded PLGA nanoparticles were analyzed spectroscopically and microscopically with FT-IR, UV-vis, and scanning electron microscopy as well as thermogravimetric analysis, Raman, and x-ray diffraction. The in vitro release of 21 from the nanoparticles exhibited a controlled release profile just over one month following a burst release in the initial six hours and in addition to this a total release ratio of %50 and %85 were obtained at pH 7.4 and 5.5, respectively. 21-loaded PLGA nanoparticles displayed remarkably effective anticancer activity than 21. The IC50 values were determined as IC50 (21-loaded PLGA nanoparticles): 0.42 +/- 0.01 mg ml-1 and IC50 (free 21 molecule): 5.74 +/- 3.82 mg ml-1 against MCF-7 cells, and as IC50 (21-loaded PLGA nanoparticles): 0.77 +/- 0.12 mg ml-1 and IC50 (free 21 molecule): 1.32 +/- 0.31 mg ml-1 against HeLa cells after the incubation period of 24 h. Our findings indicated that triazole-substituted coumarins may be used as an anticancer agent by integrating them into a polymeric drug delivery system providing improved drug loading and effective controlled drug release.

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